NADPH oxidase (NOX) inhibition attenuates acute Ca²⁺ - but not Zn²⁺-induced ROS production.

<div><p>HEt-loaded cultures were exposed to 100 μM NMDA (30 min) or 300 μM Zn²⁺/pyrithione (5 min) alone (red) or after pre-treatment with and in the presence of apocynin (500 µM), or in glucose-free media supplemented with pyruvate (15 mM) (blue) as described (see Materials and Methods). </p> <p><b>A</b>: Representative images of selected fields of neuronal cultures (“Brightfield”), and pseudocolor images (400x) of HEt fluorescence from these neurons before and 30 min following onset of exposure to NMDA (left) or Zn²⁺/pyrithione (right). The pseudocolor bar shows the 12-bit fluorescence intensity range.</p> <p><b>B</b>: Traces show time course of HEt ∆F, normalized to baseline values (F_x/F₀). Dashed lines show linear extrapolation of baseline. Traces show mean ± SD values from 4 experiments. </p> <p><b>C</b>: Quantification of HEt ∆F changes. Values show F_x/F₀ increases after subtraction of the extrapolated baseline value, 30 min after onset of the exposure. ∆F values each represent means (± SEM) of the 4 experiments; * indicates difference from NMDA alone (p< 0.001) by 2-tailed t test. </p>