Phylogenic comparisons of juvenile hormone epoxide hydrolase protein sequences of <i>H. vitripennis</i> and those other insect orders.

<p>The evolutionary relationship of juvenile hormone epoxide hydrolase (JHEH) of <i>H. vitripennis</i> and JHEH sequences from other insects. Transcript #8298 of <i>H</i>. <i>vitripennis</i> was transiently expressed as a recombinant protein using a baculovirus expression vector and biologically characterized (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0081681#pone-0081681-g009" target="_blank">Figure 9</a>). SDS-PAGE analysis of microsomes prepared from insect High Five cells infected with the recombinant baculovirus: lane 1, 2.1 µg; lane 2, 4.2 µg; lane 3, 8.4 µg, and lane M, protein size markers (SeeBlue Plus2, Invitrogen). The size of the markers is shown to the right in kDa. The arrowhead indicates the migration of the recombinant 52.1 kDa protein.