Mipu1 over-expression enhances the cytoprotection of HIF-1α against oxidative stress in H9C2 cells.

<p>“Neo”: pcDNA3.1 vector transfection group; “HIF”: pcDNA3.1-HIF-1α plasmid tranfection; “HIF+Mipu1”: cells cotransfected with pcDNA31-HIF-1α and pcDNA31-Mipu1 plasmids. <b>A</b>: immunoblotting showing the expression of Mipu1 and HIF-1α (n=3), bottom panel showing the semi-quantitative analysis of the Mipu1 and HIF-1α band against β-actin, * <i>p</i><0.05 versus “Neo”, # <i>p</i><0.05 versus “HIF” group. <b>B</b>: Colorimetric Assay showing the activity of Caspase-9 (n=4). * <i>p<0.05</i> versus H₂O₂-untreated cells, # <i>p</i><0.05 versus H₂O₂-treated “Neo” group, a <i>p<0.05</i> versus H₂O₂-treated “HIF” alone group; <b>C</b>: flowcytometry detecting the hypoploid cells, * * <i>p</i><0.05 versus H₂O₂-untreated cells, # <i>p</i><0.05 versus H₂O₂-treated “Neo” group, a <i>p</i><0.05 versus H₂O₂-treated “HIF” group, (N=5);<b> D:</b> LDH release rate, * <i>p</i><0.05 versus H₂O₂-untreated cells, # <i>p</i><0.05 versus H₂O₂-treated “Neo”group, a <i>p</i><0.05 versus H₂O₂-treated “HIF” group, n=5.