Exposure to SRM1649b augments IL-17 expression <i>in</i><i>vivo</i> and <i>in</i><i>vitro</i>.

<p>A: Male C57BL/6 mice (B6) were exposed every 3 days to SRM1649b or control PBS (400 μg/20μl PBS) by intranasal administration. 24 hours after the final treatment, mice were euthanized and total RNA was isolated from the right lung. qRT-PCR was performed to measure changes in the steady state expression of IL-17A and Cyp1a1 mRNA. B-C: Splenocytes from male B6 mice were stimulated <i>in </i><i>vitro</i> with dendritic cells isolated from B6 or Balb/c splenocytes in the presence or absence (Control) of either FICZ (200 nM) or SRM1649b (40 μg/ml). After 4 days, supernatants were harvested and the concentration of IL-17A and IFN-γ was measured by cytometric bead array. Total RNA was also isolated from the cultured cells and the relative changes in IL-22 and Cyp1b1 mRNA expression was measured by qRT-PCR. Data from 3 separate experiments were pooled and statistical comparison was made between controls and experimental cultures. *, p < 0.05; **, p < 0.01;.