Effects of miR-17 family in BMP2 mediated p-Smad induction.

<p>(<b>A</b>) Time course of p-Smad protein levels in primary cortical neurons under BMP2 (10 ng/ml) treatment: representative Western blot (upper panel) and quantitative analysis of p-Smad level normalized by Smad (bottom panel). **p < 0.01, vs 0 h. (<b>B</b>) Protein analysis of p-Smad in different groups of primary culture at 3 h after BMP2 stimulation: representative Western blot (upper panel) and quantitative analysis of p-Smad level normalized by Smad (bottom panel). *p < 0.05, **p < 0.01, vs Scramble. (<b>C</b>) Time course of luciferase reporter activity in SH-SY5Y cells under BMP2 (10 ng/ml) treatment. **p < 0.01, vs 0 h. (<b>D</b>) Luciferase reporter activity of different groups of SH-SY5Y cells at 3 h after BMP2 stimulation. *p < 0.05, **p < 0.01. Data shown are the mean ± SD for three independent experiments.