qRT-PCR analysis of miRNAs dysregulated in TNF-α treated C₂C₁₂ myotubes.

<p>Cells were treated with 2 or 20 ng/ml of TNF-α for 2 h or 4 days and the expression of miR-494, miR-690, miR-101b, miR-467b, miR-467f and miR-331 was measured by qRT-PCR. U6 RNA was used as a normalization control. The values are expressed as the means ± SEM. *,P<0.05; **,P<0.01; ***,P<0.001 versus vehicle-treated control.