Reductions in parvalbumin (PV) positive interneurons in the hippocampus of the wobbler mice.

<p>(A-D) Parvalbumin (PV) positive interneurons (illustrated by arrows) were immunohistochemically stained and counted in slices of the hippocampal formation of presymptomatic (P18-P19) wobbler mice (B) and controls (A). Symptomatic (P56) wobbler mice (D) and controls (C) were also stained. Scale bars represent 200 µm. (E) During the presymptomatic phase (P18-P19) no significant differences were found in the number of parvalbumin positive interneurons per slice in any area of the hippocampal formation between wobbler and control mice. (F) At the symptomatic phase (P56) a reduction was found in all examined areas, except in the hilus, in the wobbler mice. (G) A reduction in parvalbumin positive interneurons is seen in both wobbler mice and control mice between P18-P19 and P56; however the reduction in the wobbler mice was greater in most areas. (H) The distribution of the parvalbumin positive interneurons at the different areas are similar in wobblers and controls at the presymptomatic phase (P18-P19), and remain similar at the symptomatic phase (P56), t-test: P>0.05. (P18-19: control: n = 46 slices/4 mice, wobbler: n = 48 slices/4 mice. P56: control: n = 34 slices/4 mice, wobbler: n = 31 slices/3 mice). T-test: *P<0.05; **P<0.01. Error bars represent SEM.