Effect of flavopiridol on CFA-elicited hyperplasia, GFAP increase, persistent mechanical allodynia and thermal hyperalgesia.

<p>Flavopiridol (5 and 15 nmol) was administered by i.c.v. injection into the left lateral ventricle of the mice twice a day (at 9:00 and 19:00) after CFA treatment. Hyperplasia of paw tissue was measured by means of a digital caliper (A). Representative Western blots of GFAP and GAPDH levels in the hypothalamus after CFA injection with or without flavopiridol are shown (B). Mechanical allodynia was evaluated using von Frey filaments (C). Thermal hyperalgesia of the hind paw was assessed using the plantar test (D); data, mean ± S.E.M.; Saline (n = 6), CFA (Day 7) (n = 6), CFA (Day 7)+flavopiridol 5 nmol (n = 6), CFA (Day 7)+flavopiridol 15 nmol (n = 6); ^#<i>p</i><0.05, ^##<i>p</i><0.01, ^###<i>p</i><0.001, compared with saline; * <i>p</i><0.05, *** <i>p</i><0.001, compared with CFA (Scheffe's test).