Histological analysis.

<p>(A) Hematoxylin and eosin (H&E) stained 5 µm thick section from a representative tumor, (B) corresponding overlaid bright field and fluorescence field images showing the tdTomato red fluorescence hypoxic regions, (C) overlay of H&E section with fluorescence field image, (D) overlay of corresponding SHG tile scan of Col1 fibers with fluorescence field image, and (E) magnified inserts from (D) displaying the Col1 fibers encircling the hypoxic regions.