Optical imaging and quantification of fiber volume and fiber distribution.

<p>(A) <i>Ex vivo</i> 1× bright field and fluorescence image overlaid showing the locations of hypoxic ROIs on the tumor section. 3D visualization of (B) hypoxic and (C) normoxic FOVs; hypoxic regions are displayed in red and Col1 fibers in green. The FOV image size was 334.91×334.91×60 µm³ with a voxel size of 0.66×0.66×1 µm³. (D) Hypoxic FOVs showed a significantly lower fiber volume as compared to normoxic FOVs (**<i>p-value</i> = 0.000106, N = 10). (E) Hypoxic FOVs have a significantly larger inter-fiber distance as compared to normoxic FOVs (*<i>p-value</i> = 0.000121, N = 10).