Glia require CerPE for axonal ensheathment.

<p>(A) A genetic dissection study of sphingolipid biosynthetic pathway shows that glial knockdown of <i>Spt-I</i>, <i>schlank</i>, <i>Des1</i> and <i>Pect</i> result in swelling and wrapping defects (<i>repo-GAL4</i>) similar to the <i>lace</i> phenotype. Merged projections of confocal stacks are presented. Scale 20 µm. (B) Sphingolipid biosynthesis pathway. (C) RNAi against these 4 genes were expressed in neurons by using <i>elav-GAL4</i> driver line. HRP (red) is used to visualize the membrane morphology of the neurons. Scale 20 µm. (D) Quantification (mean± SD) showed that the swellings were only observed upon glial specific knockdown but not in neuron-specific knockdown.