Oligomerization of LipL41.

<p>(A) SDS-PAGE (15%) analysis of LipL41. The molecular weight markers (M) are shown. (B) Size-exclusion chromatography of LipL41. LipL41 was analyzed by Superdex 200 and a single peak after the void volume (dot line) was observed. The retention volume of a standard protein (thyroglobulin, 669 kDa) is indicated. (C) The sedimentation coefficient of LipL41 by analytical ultracentrifugation. The sedimentation coefficient of LipL41 was determined to be a value of 35S. (D) Dynamic light scattering analysis of LipL41. LipL41 was shown to be monodisperse with a polydispersity (%Pd) of 11.5%. The equivalent hydrodynamic radius (R_H) was 12.7 nm. The estimated molecular weight was calculated to be 1296 kDa.