Cluster widths for changing parameters and experimental verification.

<p><b>A–D</b> Full width at half maximum of Cdc42 density as obtained in <a href="http://www.ploscompbiol.org/article/info:doi/10.1371/journal.pcbi.1003396#pcbi-1003396-g002" target="_blank">Figure 2</a> for different Cdc24 (A) and Bem1 (B) concentrations, as well as for different Cdc42 extraction (C) and hydrolysis (D) rates. Arrows indicate the corresponding control cell values. <b>E</b> Examples of Cdc42 cluster density calculated for different hydrolysis rates. <b>F, G</b> Images and quantification of GFP-Cdc42 cap widths in control and Δ<i>bem2</i> cells in the presence (F, unpaired t-test with Welch correction, p<0.001) or absence (G, unpaired t-test with Welch correction, p = 0.01) of latrunculin B. Broad caps are also seen in cells expressing a slowly hydrolyzing GFP-Cdc42^G60A mutant. Images and cap widths were acquired 30–50 min after release from G1 arrest. Bar graphs correspond to mean ± SEM. Scale bars: 4 µm.