ALFA-1 is the orthologue of C9ORF72 in <i>C. elegans</i>.

<div><p>(A) Protein sequence alignment using Clustal W and BoxShade of C9ORF72 isoform 1 and ALFA-1 isoform 1. Overall, these sequences share 26% identify and 59 % similarity. </p> <p>(B) ALFA-1 has two predicted transcripts and the <i>ok3062</i> deletion mutation spans exons 3 and 4 for both transcripts.</p> <p>(B) RT-PCR confirming the complete loss of expression of the <i>alfa-1</i> transcripts. <i>act-3</i> was used as a control.</p>