Cre expression in GluA4fl/fl:Cx57+/Cre mice leads to reduced GluA4 immunoreactivity in the outer retina.

<p><b><i>A</i></b>, Generation of the conditional GluA4-deficient mice by crossing Cx57+/Cre mice with GluA4fl/fl mice <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0083076#pone.0083076-Fuchs1" target="_blank">[22]</a>. Expression of Cre recombinase leads to Cre-mediated ablation of the GluA4 gene only in Cx57-positive cells. <b><i>B-D</i></b>, Confocal projections of the OPL and distal INL from a GluA4fl/fl:Cx57+/Cre mouse labeled for calbindin (magenta; B, D) and Cre recombinase (green; C, D). Each calbindin-positive horizontal cell is also positive for Cre recombinase. Arrowheads point to blood vessels labeled by unspecific binding of the secondary anti-mouse antibody. <b><i>E-J</i></b>, Double labeling for calbindin (magenta) and GluA4 (green) in GluA4fl/fl (E-G) and GluA4fl/fl:Cx57+/Cre mice (H-J) confirmed the selective deletion of GluA4 in the OPL (arrows) but not the IPL of GluA4fl/fl:Cx57+/Cre mice. OPL/IPL, outer/inner plexiform layer; INL, inner nuclear layer; GCL, ganglion cell layer. Scale bars: B-D, 40 µm; E-J: 20 µm.