Gene expression analysis of candidate epigenetic reprogramming factors in hESCs and hiPSCs.

<p>The expression of DNA methyltransferases (DNMTs) was analyzed in undifferentiated (D0 for Day 0) as well as Day 7 (D7) and Day 14 (D14) differentiated (<b>A</b>) human embryonic stem cells (hESC; H9) and (<b>B</b>) human induced pluripotent stem cells (hiPSCs; clone 2) from normal adult human dermal fibroblasts (HUF5) by microfluidic Quantitative-PCR (Q-PCR). Cycle threshold (Ct) values were normalized to the two most stable housekeeping genes and graphed as shown. (<b>C</b>) Similar Q-PCR analysis of each histone-modifying enzyme class in H9 and (<b>D</b>) HUF5 clone 3. Note the high levels of DNMT3B, AURKB and <i>PRMT5</i> expression in undifferentiated hESCs and hiPSCs that decreases with differentiation and the elevated expression of SETD7 in D14 differentiated H9 cells and the original HUF5 fibroblasts to suggest that induction of DNMT3B, AURKB and PRMT5 expression in conjunction with SETD7 silencing may reprogram adult fibroblasts to a more hESC-like state.