Assessment of antigen-specific regulatory function in antigen-immunized mice.

<p>Inhibition of CD4⁺CD25⁻ effector T-cell proliferation by CD4⁺CD25⁺ regulatory T–cells isolated from control (KLH–immunized) mice (A,B,F) and peptide-immunized mice when the MOMP- peptide (A,C,F), the Omp5-pepide (A,D,F), and the combined peptide (A,E,F) were used as antigens. Proliferation of effector cells isolated from immunized mice alone is indicated in the leftmost bar of each group. Addition of Treg cells to T-effector cells at different ratios was also shown. Data are expressed as mean of 6 analyses ± SEM.