The effects of citicoline on Hypoxia/OGD-induced disruption of TJPs expression in endothelial cells.

<p>Representative images of TJP staining on endothelial cells. Green: ZO-1/occluding/claudin-5; Red: F-actin; Blue: nuclear. Scale bars: 50 µm. Hypoxia/OGD decreases the expression and results in a discontinuous distribution of ZO-1 and occludin on the membrane of HUVECs (<b>A</b>). Exposure to hypoxia causes a decreased expression and discontinuous distribution of ZO-1 and claudin-5 on the membrane of bEnd.3s (<b>B</b>). Treatment of citicoline restores the linear distribution of ZO-1, occludin and claudin-5.