ATG4C and BECN1 were targets of <i>MIR376A</i>.

<p>(A and B) <i>MIR376A</i> mature miRNA sequence and <i>MIR376A</i> binding target sequences in the 3' UTR of <i>ATG4C</i> (A) and <i>BECN1</i>(B) mRNAs were depicted. <i>MIR376A</i> seed sequence was shown in bold letters and and complemetary sequences were indicated. (C and D) qPCR analysis of <i>ATG4C</i> and <i>BECN1</i> mRNA expression in control miRNA (<i>MIR-CONT</i>) or <i>MIR376A</i> overexpressing cells under non-starved (NON STV) or starved (STV) condition (mean ± S.D. of independent experiments, n = 3, *p<0,05). (E and F) Immunoblots showing ATG4C and BECN1 protein levels following control miRNA (<i>MIR-CONT</i>) or <i>MIR376A</i> overexpression. ACTB was used as a loading control. ATG4C/ACTB or BECN1/ACTB densitometric ratios were marked.