The effect of transfection of odontoblast-like cells with siRNA on the levels of MMP-3 activity.

<p>Effects of the combination of IL-1β treatment (0, 0.25, 2.5 and 25 ng/mL) on MMP-3 activity released from cultured cells 24 h after transfection with siRNA. The cells were incubated in serum-free medium in the absence or presence of exogenous IL-1β at the concentrations indicated for either 6 h (white bars) or 12 h (black bars). Data are presented as the mean ± SD of four independent experiments. *, ^# and ^† denote significant differences; (*<i>P</i><0.05 <i>vs</i>. control; ^#<i>P</i><0.05 <i>vs</i>. control siRNA; ^†<i>P</i><0.01, as indicated by the bracket).