Use of a multi-classifier approach to predict the function of a novel protein.

<p>C10orf104 was a novel protein associated with mitotic chromosomes whose amino acid sequence offered no clues to its functions. When its association with isolated mitotic chromosomes was investigated using five different types of proteomic experiments, the protein was shown to cluster with APC/C components <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.1001742#pbio.1001742-Ohta1" target="_blank">[4]</a>. This led us to predict that C10orf104 might be a novel APC/C component—a prediction that was confirmed by studies carried out independently in three other groups <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.1001742#pbio.1001742-Kops1" target="_blank">[24]</a>–<a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.1001742#pbio.1001742-Hubner1" target="_blank">[26]</a>. The protein is now known as APC16.