Diabetes-induced increases in ROS are suppressed in NOX2^-/- chimeras.

<p><b>A</b>. Real time DHE imaging of superoxide formation in retinas from diabetic WT→WT chimeras mice show a strong reaction to DHE as compared with control WT→WT chimeras, the reaction is almost completely abolished in diabetic NOX2^-/-→WT and WT→NOX2^-/- chimeras. Diabetes-induced increase in superoxide production is also abolished by pretreatment with SOD (400U/mL) or apocynin (1 mM). GCL = ganglion cell layer, INL = inner nuclear layer, ONL = outer nuclear layer. <b>B</b>. Quantitative analysis of fluorescence intensity in DHE images shows a significant increase in superoxide formation in diabetic WT→WT chimeras. Superoxide production is significantly reduced in diabetic WT→NOX2^-/- and NOX2^-/-→WT chimeras as compared with the diabetic WT→WT chimeras. Pretreatment with apocynin or SOD blocked the DHE reaction. Data represent mean <u>+</u> S.E., *=P<0.001 vs. WT→WT control mice. #=P<0.001 vs WT→WT diabetic mice, n=4, scale bar = 20 μm.