Autophagy inhibition facilities salinomycin-induced apoptosis in osteoblastoma cells.

<p>U2OS cells were treated with vehicle (0.1 % of DMSO), or 10 μM of salinomycin in the presence or abscess of 3-MA (1 mM) for 32 hours, Annexin V positive cells were sorted by FACS (A), quantified results were shown in (B). U2OS cell apoptosis was also detected by Histone-DNA ELISA assay as described (C), some of the cells were also pretreated z-VAD-fmk (50 μM) for 2 hours (B and C). U2OS cells were pre-treated with z-VAD-fmk (50 μM) for 2 hours, followed by salinomycin (10 μM) plus 3-MA (1 mM) co-administration, after 48 hours of culture, cell viability was analyzed (D). Scramble or LC3B siRNA (100 nM each) transfected HEK-293T cells were treated with salinomycin (Sali, 10 μM) for 32 hours, cell apoptosis was analyzed by histone-DNA ELISA assay (E). The effect of 3-MA (0.5 or 1 mM) on salinomycin (Sali, 10 μM, 32 hours)-induced MG-63 cell apoptosis was also shown (F and G). Experiments in this figure were repeated three times, similar results were obtained. *<i>p</i><0.05.