Effects of SOCE inhibition on mitochondrial complex I activity and mitochondrial biogenesis.

<p>PC12 cells were trasfected with STIM1 specific siRNA (Si-STIM1) or control siRNA (Si-Control) 72 h before MPP⁺ insult, or pretreated with ML-9 (50 μM) or MRS (15 μM) 30 min before MPP⁺ insult, and the activity of mitochondrial respiratory chain complex I was detected (A). Mitochondrial DNA content was determined by quantitative real-time PCR by comparing the mitochondrially encoded D-loop and ATP8 gene to a nuclear-encoded GAPDH gene (B), and the mRNA expression of mitochondrial biogenesis factors were measured by real-time RT-PCR (C). The data were represented as means ± SD from five experiments. <b><i>*</i></b><i>p</i> < 0.05 vs. MPP⁺ alone. <b><i>#</i></b><i>p</i> < 0.05 vs. control siRNA. n.s., not statistically significant.