Notch3-shRNA weakened the inhibitory effect of CHIR99021 on apoptosis in H460 but not in A549 and H157.

<p>(A) Cell apoptosis was analyzed by flow cytometry. The cells in Q2 and Q4 represent late and early apoptosis cells, respectively. (B) Histogram represents the percentages of early and late apoptosis cells. (C) Real-time RT-PCR was performed to evaluate the expression of caspase-3. (D) Western blot panels represent the expression of caspase-3. The histogram of densitometric analysis is shown on right. Error bar represents mean ± s.d.