The processes of rod-microglia penetrates the IPL.

<p>Iba-1 and NF-200 immunostaining. Retinal whole-mounts. Using the z stack tool associated with the microscope, we observed that some processes of rod-microglia penetrate the ganglion-cell layer and inner plexiform layer (A). The pressure observed by the cover slip on the whole-mount caused retinal-like section effect on one edge of the tissue that revealed that, in the IPL, rod-microglia were apparently in close relation with the dendrites of NF-200+RGCs (B1,B3). (INL: inner plexiform layer; IPL: inner plexiform layer; GCL: ganglion-cell layer; NFL: nerve-fibre layer; RGC: retinal ganglion-cell layer; asterisk: soma; arrow: rod-microglia processes).