Hif-2α has opposing effects on bacterial burden than Hif-1α.

<p>(A) Bacterial pixel counts of dominant active <i>hif-2αa</i> (DA2) bacterial burden levels in 4 dpi infected embryos compared to phenol red (PR) and dominant active <i>hif-1αb</i> (DA1) injected controls. Data shown are mean ± SEM, n = 52–79 as accumulated from 3 independent experiments. (B) Example fluorescence micrographs of the data shown in (A). (C) Bacterial pixel counts of dominant negative <i>hif-2αa</i> (DN2) bacterial burden levels in 4 dpi infected embryos compared to phenol red (PR) and dominant active <i>hif-1αb</i> (DA1) injected controls. Data shown are mean ± SEM, n = 74–82 performed as 3 independent experiments. (D) Example fluorescence micrographs of the data shown in (C). (E) L-plastin (macrophages and neutrophils) and TSA (neutrophils only) wholebody counts at 30 hpf after injection of dominant active (DA2) and dominant negative (DN2) <i>hif-2αa</i> RNA. No significant difference was observed between groups. Data shown are mean ± SEM, n = 77–82 as accumulated from 3 independent experiments. (F) L-plastin and TSA wholebody counts at 120 hpf after injection of dominant active (DA2) and dominant negative (DN2) <i>hif-2αa</i> RNA. Data shown are mean ± SEM, n = 87–90 as accumulated from 3 independent experiments.