Effect of SopB R36 and R42 mutations on mini-F stability.

<p><b>A</b>. <i>sopAB</i> operon and <i>sopC</i> in natural configuration (<i>cis</i>; see sketches): white bars - strain DLT1900 carrying pDAG218 and derivatives, grown at 30°C in minimal-glycerol-leucine medium (t∼160 min); shaded bars - strain DH10B carrying pDAG173 and derivatives, grown in minimal-glycerol-Casamino acids medium (t∼80 min). Error bars are standard deviations of ≥3 loss-rate assays. Note 10-fold scale change for <i>sopC</i>⁺ plasmids. <b>B</b>. <i>sopAB</i> and <i>sopC</i> on separate plasmids, pAM138 and mini-F respectively (<i>trans</i>), in strain MC1061 grown in minimal-glycerol-leucine medium: white bars – loss rates. Shaded bars show ATPase deficits as the inverse of ATPase stimulation by each SopB <i>in vitro</i>, determined previously <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1003956#pgen.1003956-AhSeng1" target="_blank">[12]</a>.