MSCRAMMs are required for agglutination.

<p>The tube-based agglutination assay was performed and quantified with LAC-WT and mutant strains. <b>A</b>) LAC-WT compared to a sortase mutant (LAC Δ<i>srtA</i>; 26% agglutination). <b>B</b>) LAC-WT compared to mutations of individual MSCRAMMs (<i>clfA</i>, <i>clfB</i>, and <i>fnbAB</i>) at 1.5 hr post HP addition. The <i>clfA</i> mutant displayed 55% agglutination. <b>C</b>) LAC-WT compared to mutants in protein A (<i>spa</i>) and <i>ica</i> (encoding PIA) at 2 hr post HP addition. Bars are standard deviations of at least three replicates, and significant differences (p<0.001) as determined by Student's <i>t</i>-test are indicated by “*”.