F508del bone marrow-derived cells, specifically neutrophils, play an important role in mediating thrombocytopenia.

<p>A–B. Wildtype mice were irradiated and then reconstituted with wildtype or <i>F508del</i> bone marrow to create chimeric mice. At 24 h after LPS challenge, wildtype mice receiving <i>F508del</i> bone marrow developed thrombocytopenia (A) (N = 9 in each group) with higher plasma TXB2 levels (B) (N = 5 in each group) compared to wildtype mice receiving wildtype marrow. C–D. Wildtype mice were given anti-Gr-1 antibody to deplete neutrophils and then reconstituted with either wildtype or <i>F508del</i> neutrophils. 24 h after LPS challenge, wildtype mice receiving <i>F508del</i> neutrophils developed thrombocytopenia (C) and had higher levels of plasma TXB2 (D). N = 5 in each group. Data were analyzed by t-test and presented in mean ± SD. P values are listed on each panel.