Western Blot analysis for the detection of HA and NP.

<p>Cell lysates were harvested at indicated hours post infection (hpi) with <b>(A, C)</b> D1701-V-HAh5n (moi 3.0) or <b>(B, D)</b> D1701-V-NPh5 (moi 1.0). As controls non-infected cells (lanes ni) or parental D1701-V- (moi 1.0) infected cells (lanes V) were tested at 24 hpi. <b>(A)</b> H5 HA was detected with the specific mAb 15A3 (diluted 1∶5,000), <b>(B)</b> NP was detected with specific mAb 2442 (diluted 1∶2,000). <b>(C)</b> The mAb 4D9 (diluted 1∶800) was used to detect the ORFV major envelope protein F1L expressed at late times pi. <b>(D)</b> Beta-actin was demonstrated as loading control. The apparent mol. wt. of the specific proteins is indicated in kilodalton (kDa).