Activation of PI3K results in phosphorylation of Akt and mTOR and inhibition of the pathway occurs via PTEN.

<p>Representative western blot bands (<b>A</b>) are shown of p-Akt and β-actin as well as densitometric analysis with a significant increase of expression in the ACM+BMP7 group and a significant decrease of expression in the ACM+BMP7+Foll group. Next, representative western blot bands (<b>B</b>) are shown of p-PTEN and β-actin with densitometric analysis showing significant decrease of expression in the ACM+BMP7 group and a significant increase of expression in the ACM and ACM+BMP7+Foll groups. Lastly, representative western blot bands (<b>C</b>) are shown of p-mTOR and total mTOR with densitometric analysis showing a significant increase of expression in the ACM+BMP7 group and a significant decrease of expression in the ACM+BMP7+Foll groups. *p<0.05 vs Control, #p<0.05 vs ACM, $p<0.05 vs ACM+BMP7, n = 5–7.