Treatment with BMP-7 decreases expression of M1 macrophage marker, iNOS, as well as pro-inflammatory cytokines.

<p>Left panel <b>A</b> shows representative photomicrographs of anti-iNOS in red (a, e, i, m), DAPI in blue (b, f, j, n), merged images (c, g, k, o) and enlarged merged images (d, h, l, p). Scale bar = 50 µm. Histogram <b>B</b> shows quantitative analysis of the percentage of cells expressing iNOS with a significant decrease in the control and ACM+BMP7 and groups and a significant increase in the ACM and ACM+BMP7+Foll groups; n = 5–6. Histogram <b>C</b> shows quantitative analysis of expression of IL-6 with a significant decrease in the ACM+BMP7 group; n = 5–6. Histogram <b>D</b> shows quantitative analysis of expression of TNF-α with also a significant decrease in the ACM+BMP7 group; n = 4–6. Histogram <b>E</b> shows quantitative analysis of MCP-1 with also a significant decrease in the ACM+BMP7 group; n = 5–7. *p<0.05 vs Control, #p<0.05 vs ACM, $p<0.05 vs ACM+BMP7.