MCT inhibition increases the magnitude of volume loss and influences the RVI response.

<p>Time course of volume changes in single fibres where MCT activity was inhibited using either phloretin (▾, n = 7 fibres from 4 muscles from 2 mice) or pCMBS (•, n = 11 fibres from 6 muscles from 3 mice), or where NKCC activity was inhibited using bumetanide (⋄, n = 39 fibres from 21 muscles from 6 mice) compared to control (▪; n = 30 fibres from 16 muscles from 8 mice). Fibres were incubated in the presence of inhibitor for 30 minutes prior to addition of NaLac to raise extracellular osmolarity by 49 mosmol/kg. pCMBS resulted in a greater volume loss and more rapid volume recovery than seen in controls or in bumetanide treated fibres. Phloretin resulted in a greater volume loss than seen in controls, bumetanide treated fibres and pCMBS treated fibers. The rates of volume recovery in pCMBS and phloretin treated fibres were similar.