Western blot analysis.

<p>Expression levels of BHMT, MS and CBS were determined by Western blot (A). MT, torpid <i>M. ricketti</i>; MA, active <i>M. ricketti</i>; R, <i>R. leschenaultii</i>. All samples are from brain tissue. Arrows indicate predicated molecular weight (kDa) of proteins. (B) Relative expression levels of the proteins are represented as mean ± SD. Statistical significance by two tailed Student’s t-tests: *<i>P</i><0.05, **<i>P</i><0.001.