Warfarin pretreatment enhances tumor Ad5ROBO4 vector expression.

<p>A. Multiorgan immunoblot of vehicle (left lanes) or warfarin (right lanes) pretreated <i>Rag2^−/−</i> mice injected with 1.0×10¹¹ vp of Ad5ROBO4. B. Densitometry of A revealed that vehicle pretreatment was associated with robust liver, detectable splenic, and trace to undetectable expression in all other sampled organs. Warfarin pretreatment produced a 2.5-fold increased splenic and a 3-fold decreased liver expression while all other organs still evidenced trace to undetectable expression. C. Immunoblot and densitometry of liver and tumor EGFP, VE-cadherin, and β-tubulin expression in vehicle (left lanes) or warfarin (right lanes) from the same pretreated, Ad5ROBO4-injected mice as in A and B. EGFP densitometry normalized to VE-cadherin, revealed a 4.7-fold decrease in liver and 2-fold increase in increase KO and SC tumor expression produced by warfarin pretreatment. A–C: representative immunoblots from n = 2 mice from 2 independent experiments.