Effects of BisGMA on the expression of PGE2 and COX-2.

<p>(A) RAW264.7 macrophages were stimulated with the indicated concentrations of BisGMA for 2 h. The supernatant was harvested and detected by ELISA assay of PGE2. Values are expressed as mean ± SD (n = 3). *represents significant difference from control values with <i>p</i><0.05. (B) Cells were exposed to the indicated concentrations of BisGMA for 2 h. Cells were harvested and protein extracts were subjected to SDS-PAGE. Values are expressed as mean ± SD (n = 3). The ratio of immunointensity between the COX-2 and β-actin was calculated. *represents significant difference from control values with <i>p</i><0.05. (C) Cells were stimulated with 3 µM BisGMA for the indicated treatment periods. Cells were harvested and protein extracts were subjected to SDS-PAGE. Western blot analysis using antibodies against COX-2 and β-actin. Values are expressed as mean ± SD (n = 3). The ratio of immunointensity between the COX-2 and β-actin was calculated. *represents significant difference from control values with <i>p</i><0.05.