Effect of PI3-kinase inhibition on the inhibitory action of apelin-13 on BK_Ca channel currents in rat middle cerebral arterial VSM cells.

<p>Whole-cell BK_Ca currents were recorded as described in the Methods. <b>A</b>-<b>D</b>: Representative tracings showing the currents recorded from a single VSM cell under the following sequential treatment conditions: control (A); followed by superfusion with 0.1 µM apelin-13 (B) for 5 min; washout of apelin-13; superfusion with LY-294002 (10 mM, 5 min) (C); and superfusion with apelin-13 plus LY-294002 (D). <b>E</b>: Average whole cell current density-voltage plots of BK_ca current. <b>F</b>: Bar graph summarizing the average current density (pA/pF) at 50 mV obtained at each treatment condition described above. Values are mean±SEM (n=6 cells). *<i>P</i><0.05 indicates a significant difference from the corresponding control value.