Type I IFN production is enhanced by pertussis toxin treatment.

<p>pDCs or CD11b⁺ monocyte/macrophages from WT or <i>Ebi2</i>^−/− littermate mice were preincubated with 100 ng/ml PTX, and then stimulated with TLR7 agonist ssPolyU (<b>A</b>), TLR9 agonist CpG-A ODN2216 (<b>B</b>) or TLR3 agonist poly(I:C) (<b>C</b>). IFN-α secretion in culture supernatants after 40 hr stimulation were measured by ELISA. Bars represent mean values of 3 independently performed experiments (white denotes WT, shaded denotes KO); circles represent individual experiments. <i>P</i>-values are denoted when considered statistically significant (<i>p</i><0.05); n.s. denotes not statistically significant. IFN-α was not detectable in the absence of stimulation.