Patterns of aberrant axon projections in <i>mid</i> mutant embryos.

<p>Anterior end is up, the midline is marked by vertical lines. Embryos in <b>panels A–E</b> were stained with Mab 22C10 to visualize vMP2 and dMP2 neurons and their projections. Arrow indicates a normal projection in wild type (A, B), arrow with star indicates an abnormal/stalled projection in the mutant from dMP2 and vMP2 neurons (panels C–E). <b>Panel F, G</b>: Embryos were double-stained for GFP (green) and Odd-skipped (Odd, red). Arrow indicates normal projection from vMP2 in wild type (F), arrow with star indicates projection from vMP2 abnormally exiting the nerve cord in the mutant (G). <b>Panels H, I, J:</b> Embryos were double-stained for GFP (green) and Eve (red). Arrow indicates normal projections from MP1 in wild type (H), arrow with star indicates abnormal MP1 projection in the mutant (I, J). <b>Panels K, L:</b> Embryos were stained for LacZ (green). Arrows indicate normal projections from pCC in wild type (K), arrow with star indicates abnormal projection from pCC in the mutant (L). <b>Panels M, N:</b> Embryos were stained for LacZ (green). Smaller arrow indicates normal motor intersegmental nerve bundle from aCC and RP2 neurons in wild type (M) and in the mutant (N).