Expression of CLCA4 correlates with epithelial differentiation.

<p><b>A</b>, HMLE cells were separated into epithelioid and mesenchymal subpopulations by differential trypsinization and subjected to RT-qPCR. The transcriptional profile reveals that CLCA4 is highest in the most trypsin-resistant fraction, correlating with E-cadherin and CLCA2. The 5 min fraction was normalized as 1. P<0.001 for all comparisons between 5 min and 20 min samples. <b>B</b>, EMT induced by TGF-beta treatment (2.5 ng/ml) downregulates CLCA4 and CLCA2. Values are normalized to no-drug control. P<0.01 for each comparison to no-drug control.