Analysis of action potential waveform in rhythmic bursting R15 from mature and old animals.

<p>A: action potentials in a single burst. B: measurements of waveform parameters (Depolarization amplitude, Depolarization Duration, Repolarization amplitude, Repolarization duration). Bar graphs showing the mean depolarization amplitude (C), the mean depolarization duration (D), repolarization amplitude (E), repolarization duration (F) of AP waveforms recorded in rhythmic R15 bursting neurons from old and mature animals, before and after 1 mM ACh application. Values are expressed as mean ± SEM. mV: milli volt; msec: milli seconds. An asterisk or a black-filled square indicates statistically significant differences. Differences were considered significant (n=4; One way ANOVA and Tukey’s HSD test) at the level of p <0.05 (asterisk) or p <0.01 (square). G & H: Representative action potentials waveforms of R15 bursting activity recorded in bM-R15 (G) and bO-R15 (H) neurons before and after 1 mM ACh application. bM-R15: rhythmic bursting R15 from mature animals; bO-R15: rhythmic bursting R15 from old animals, bM-R15 ACh and bO-R15 ACh are bM-R15 or bO-R15 neurons after ACh exposure.