Inactivation of mTORC1 by CoCl₂ and restoring by Rg-1.

<p>ARPE-19 cells were pre-treated with Rg-1 (125 µM) for 2 hrs, followed by CoCl₂ (600 µM) administration, cells were further cultured for indicated time points, phospho (p)- and total -Akt, S6 and 4E-BP1 were analyzed by Western blots (A). ARPE-19 cells were treated with RAD001 (200 nM) and rapamycin (400 nM), after 4 hrs, phospho (p)- and total (t)-Akt, S6 and p-Erk1/2 as well as tubulin were examined (B), cell viability was analyzed by MTT assay after 24 hrs (C). Western blot data were quantified as described. Data were expressed as mean±SD. <b>^#</b><b><i>p</i></b><0.05.