Conservation of typical GPCR signaling motifs in Lgr4-6.

<p>The primary amino acid sequences of 282 Class 1 rhodopsin-like GPCRs were aligned in ClustalΩ and a subset presented due to space limitations (ClustalX color view based upon degree of conservation; See <a href="http://jalview.org" target="_blank">jalview.org</a> for complete color table, briefly, Acidic (E,D: Magenta); Basic (R, K:Red); (P: Yellow). The entire sequence alignment can be downloaded from <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0084476#pone.0084476.s003" target="_blank">File S1</a> and opened in Jalview. Lgr1-3 (FSHR, LSHR, and TSHR) and Lgr4-6 are highlighted by hatched and solid boxes. (A) Primary acid alignment from a subset of GPCRs at the “DRY” motif and (B) primary acid alignment from a subset of GPCRs at the NPXXY motif. Arrow in (A) points to the conserved Arginine in the “DRY” motif. Line in (B) highlights the NPXXY motif. The position label at top indicates amino acid position from the start of the alignment. At the bottom, alignment conservation, quality, and consensus for each amino acid (+, indicates significant conservation of the site).