Analysis of MuHV-4 structural protein glycosylation.

<p>Control or deglycosylated proteins of purified MuHV-4 virions were separated by SDS-PAGE. After migration, each sample was divided in 30 serial slices along the lane and proteins of each slice were identified as described in the <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0083842#s2" target="_blank">Materials and Methods</a>. For the two treatments, slices containing M3, ORF4 (gp70), ORF8 (gB), ORF22 (gH), ORF27 (gp48), ORF28, ORF39 (gM), ORF47 (gL), ORF51 (gp150) and ORF58 proteins are shown with color intensity indicating the mean relative abundance (emPAI). For each lane, the abundances are expressed relatively to the band displaying the highest emPAI which is taken as 1. For each protein, predicted molecular mass is shown (theoretical lane). For the gB protein, the dashed slice indicated the predicted position of gB proteins before potential cleavage by cellular furin. The position of a MM standard is shown on the left.