Sorting and characterization of MP and SP cells from DPCs.
- Emilio Satoshi Hara (393519)
- Mitsuaki Ono (393520)
- Takanori Eguchi (393518)
- Satoshi Kubota (417977)
- Hai Thanh Pham (503230)
- Wataru Sonoyama (67504)
- Shoji Tajima (12397)
- Masaharu Takigawa (58830)
- Stuart K. Calderwood (191496)
- Takuo Kuboki (393521)
Publication date
December 2013
DOIAbstract
<p>A) Identification and sorting of side population (SP) and main population (MP) by FACS using Hoechst-33342 (5 μg/mL) and verapamil (100 μM) as an inhibitor of ABCG2 binding cassette. B) Quantitative analysis of colony forming ability (CFU-F assay) of SP and MP cells. Results are the mean (±S.E.M.) of quadruplicate samples. C–D) mRNA levels of <i>ABCG2</i>, <i>NANOG</i> and <i>OCT-4</i> in SP and MP cells. Results are the average (±SD) of a single experiment run in triplicate. * P<0.05, ** P<0.01, *** P<0.001, unpaired <i>t</i>-test, compared to MP.</p
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