Effects of GLA on platelet adhesion on collagen-coated surfaces.

<p><b>H</b>uman whole blood was labeled with Calcein-AM, treated with (lower channels) or without (upper channels) 0.1μg/ml (A) or 0.5μg/ml (B) of GLA, and perfused over collagen-coated surface for 30 min at a shear rate of 40 dyn/cm² (1000s^-1). Platelet adhesion and aggregates were observed under a fluorescence microscope. Photos were taken at 10min (A) after flow. Arrow indicates the flow direction. Data plotted are means ± standard errors of fluorescence intensity of three individual experiments (*P < 0.05, ***P < 0.01). The Student t test was used to calculate P values for differences.