Bead-based method allows the detection of antigen-specific B cells.

<p>(<b>A</b>) B cells purified from Tg mice were incubated with human albumin, MOG_1–125 or pp65 coated beads and the frequency of antigen specific B cells was quantified. The B cells were preincubated with soluble human Albumin, MOG_1–125 or pp65 before incubation with MOG_1–125 coated beads. Data are presented as mean ± sem <b>B</b>). B cells purified from Tg mice were preincubated with increasing doses of soluble MOG_1–125 before incubation with MOG_1–125 coated beads. The experiments were repeated 3 times and similar results were obtained.