PCA and PLS-DA of fatty acids from phospholipids present in <i>Mesorhizobium</i> N₃₃ growing at constant temperatures or exposed to suboptimal 4°C.

<p>Growth temperatures: GT21 = 21°C (control); GT4 = 4°C; GT10 = 10°C. For all data, row-wise normalization was used by a pooled averaged reference samples (GT21 or T0), and data were auto scaled and log transformed. A: PCA analysis was performed on 17 fatty acids. B: PLS-DA plot showing the compound changes (permutation test, P<0.01) at different times of exposure to suboptimal 4°C. T0 = 21°C (reference), T1 = 2 min; T2 = 4 min; T3 = 8 min; T4 = 60 min; T5 = 240 min to 4°C of cells grown at 21°C. The optimal PLS-DA model for fatty acids from phospholipids used the top five component with a Q² = 0.74.