Murine model of renal damage induced by hyperlipidemia.

<p>(<b>A</b>) Serum cholesterol levels from C57BL/6 (WT) and ApoE knockout (ApoE) mice fed a normal chow diet (ND) or a hyperlipidemic diet (HC) for 5 or 10 weeks. (<b>B</b>) Semiquantitative assessment and representative secondary ion images under the irradiation of bismuth cluster showing lipid content in kidneys from mice at 10 weeks of dietary intervention. In order to normalize tissue size variation, metabolite measurements were expressed as % relative intensity = ratio between the metabolite area and total tissue area (phosphate area) (<b>C</b>) Representative Masson trichrome staining in mouse kidneys and semiquantitative assessment of lesions in glomerular, tubular and interstitial compartments. Original magnification x 100. *p<0.05 vs WT with ND in each time-period, † p<0.05 vs WT with HC in each time-period, ‡ p<0.05 vs ApoE knockout mice with ND in each time-period. Values shown are mean±SD, n = 8 per group.